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KMID : 0545120110210101069
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Journal of Microbiology and Biotechnology
2011 Volume.21 No. 10 p.1069 ~ p.1072
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Monitoring of Leuconostoc Population During Sauerkraut Fermentation by Quantitative Real-Time Polymerase Chain Reaction
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Kim So-Young
Yoo Ki-Seon
Kim Yu-Jin
Seo Eun-Young
Kim Beom-Soo
Han Nam-Soo
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Abstract
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A real-time PCR assay method was established to monitor Leuconostoc spp. populations via specific amplification of the dextransucrase gene. Quantification of L. mesenteroides B-512F using both genomic DNA and cell suspensions yielded a log-linear correlation spanning approximately 5 log units. By using this method, monitoring changes of Leuconostoc spp. during sauerkraut fermentation was successfully accomplished with accuracy after inoculation of starter and sugars (sucrose and maltose).
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KEYWORD
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sauerkraut, Leuconostoc, real-time PCR, monitoring, dextransucrase
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